diff --git a/changelog.d/6646.misc b/changelog.d/6646.misc
new file mode 100644
index 0000000000..0f2e3f0881
--- /dev/null
+++ b/changelog.d/6646.misc
@@ -0,0 +1 @@
+[App Layout] Obsolete settings are not shown when App Layout flag is enabled
diff --git a/vector/src/main/java/im/vector/app/features/settings/VectorPreferences.kt b/vector/src/main/java/im/vector/app/features/settings/VectorPreferences.kt
index cefbe64d9d..d60d0ea73a 100755
--- a/vector/src/main/java/im/vector/app/features/settings/VectorPreferences.kt
+++ b/vector/src/main/java/im/vector/app/features/settings/VectorPreferences.kt
@@ -165,6 +165,11 @@ class VectorPreferences @Inject constructor(
         const val SETTINGS_LABS_AUTO_REPORT_UISI = "SETTINGS_LABS_AUTO_REPORT_UISI"
         const val SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME = "SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME"
 
+        /**
+         * This is not preference, but category on preferences screen which contains [SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME].
+         * Needed to show/hide this category, depending on visibility of [SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME]. */
+        const val SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME_CATEGORY = "SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME_CATEGORY"
+
         private const val SETTINGS_DEVELOPER_MODE_PREFERENCE_KEY = "SETTINGS_DEVELOPER_MODE_PREFERENCE_KEY"
         private const val SETTINGS_LABS_SHOW_HIDDEN_EVENTS_PREFERENCE_KEY = "SETTINGS_LABS_SHOW_HIDDEN_EVENTS_PREFERENCE_KEY"
         private const val SETTINGS_LABS_ENABLE_SWIPE_TO_REPLY = "SETTINGS_LABS_ENABLE_SWIPE_TO_REPLY"
diff --git a/vector/src/main/java/im/vector/app/features/settings/VectorSettingsLabsFragment.kt b/vector/src/main/java/im/vector/app/features/settings/VectorSettingsLabsFragment.kt
index eb7864a89d..c1253f4ab4 100644
--- a/vector/src/main/java/im/vector/app/features/settings/VectorSettingsLabsFragment.kt
+++ b/vector/src/main/java/im/vector/app/features/settings/VectorSettingsLabsFragment.kt
@@ -27,6 +27,7 @@ import im.vector.app.R
 import im.vector.app.core.preference.VectorSwitchPreference
 import im.vector.app.features.MainActivity
 import im.vector.app.features.MainActivityArgs
+import im.vector.app.features.VectorFeatures
 import im.vector.app.features.analytics.plan.MobileScreen
 import im.vector.app.features.home.room.threads.ThreadsManager
 import org.matrix.android.sdk.api.settings.LightweightSettingsStorage
@@ -39,6 +40,7 @@ class VectorSettingsLabsFragment :
     @Inject lateinit var vectorPreferences: VectorPreferences
     @Inject lateinit var lightweightSettingsStorage: LightweightSettingsStorage
     @Inject lateinit var threadsManager: ThreadsManager
+    @Inject lateinit var vectorFeatures: VectorFeatures
 
     override var titleRes = R.string.room_settings_labs_pref_title
     override val preferenceXmlRes = R.xml.vector_settings_labs
@@ -72,6 +74,10 @@ class VectorSettingsLabsFragment :
                 true
             }
         }
+
+        findPreference<VectorSwitchPreference>(VectorPreferences.SETTINGS_LABS_UNREAD_NOTIFICATIONS_AS_TAB)!!.let {
+            it.isVisible = !vectorFeatures.isNewAppLayoutEnabled()
+        }
     }
 
     /**
diff --git a/vector/src/main/java/im/vector/app/features/settings/VectorSettingsPreferencesFragment.kt b/vector/src/main/java/im/vector/app/features/settings/VectorSettingsPreferencesFragment.kt
index 0bd5316b8f..458ba79ee3 100644
--- a/vector/src/main/java/im/vector/app/features/settings/VectorSettingsPreferencesFragment.kt
+++ b/vector/src/main/java/im/vector/app/features/settings/VectorSettingsPreferencesFragment.kt
@@ -28,9 +28,11 @@ import im.vector.app.core.dialogs.PhotoOrVideoDialog
 import im.vector.app.core.extensions.restart
 import im.vector.app.core.preference.VectorListPreference
 import im.vector.app.core.preference.VectorPreference
+import im.vector.app.core.preference.VectorPreferenceCategory
 import im.vector.app.core.preference.VectorSwitchPreference
 import im.vector.app.features.MainActivity
 import im.vector.app.features.MainActivityArgs
+import im.vector.app.features.VectorFeatures
 import im.vector.app.features.analytics.plan.MobileScreen
 import im.vector.app.features.settings.font.FontScaleSettingActivity
 import im.vector.app.features.themes.ThemeUtils
@@ -44,6 +46,7 @@ class VectorSettingsPreferencesFragment :
 
     @Inject lateinit var vectorPreferences: VectorPreferences
     @Inject lateinit var fontScalePreferences: FontScalePreferences
+    @Inject lateinit var vectorFeatures: VectorFeatures
 
     override var titleRes = R.string.settings_preferences
     override val preferenceXmlRes = R.xml.vector_settings_preferences
@@ -99,6 +102,10 @@ class VectorSettingsPreferencesFragment :
             }
         }
 
+        findPreference<VectorPreferenceCategory>(VectorPreferences.SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME_CATEGORY)!!.let { pref ->
+            pref.isVisible = !vectorFeatures.isNewAppLayoutEnabled()
+        }
+
         // Url preview
         /*
         TODO Note: we keep the setting client side for now
diff --git a/vector/src/main/res/xml/vector_settings_preferences.xml b/vector/src/main/res/xml/vector_settings_preferences.xml
index a3b8a3476c..7700de6986 100644
--- a/vector/src/main/res/xml/vector_settings_preferences.xml
+++ b/vector/src/main/res/xml/vector_settings_preferences.xml
@@ -30,7 +30,9 @@
 
     </im.vector.app.core.preference.VectorPreferenceCategory>
 
-    <im.vector.app.core.preference.VectorPreferenceCategory android:title="@string/spaces">
+    <im.vector.app.core.preference.VectorPreferenceCategory
+        android:title="@string/spaces"
+        android:key="SETTINGS_PREF_SPACE_SHOW_ALL_ROOM_IN_HOME_CATEGORY">
 
         <im.vector.app.core.preference.VectorSwitchPreference
             android:defaultValue="false"